This work presents the pathology description, isolation and identification of canine herpesvirus\n(CHV-1) in Mexico, a virus that causes a generalized hemorrhagic infection in puppies from the canidae\nfamily. Methods: Isolates were obtained from puppies that died within the first four weeks\nof life and had lesions consistent with canine herpesvirus. Results: The main gross lesions were\npetechial and ecchymotic hemorrhages in kidneys, liver and lungs; proliferative interstitial nephritis;\nmultifocal necrosis in liver and kidneys; and encephalitis with intranuclear inclusion bodies.\nHerpesvirus was confirmed through direct immunofluorescence, electron microscopy and polymerase\nchain reaction for DNA polymerase and glycoprotein B genes. Discussion: Eight strains\nwere isolated and identified as canine herpesvirus corresponding to three of the working cases\nwith gross and microscopic lesions very similar to those described in the literature; then, isolates\nwere confirmed by PCR gene amplification, positive reactions on immunofluorescence and observations\nfrom electron microscopy. This work represents the first report of this disease, including\ngross and histological lesions, and confirmation by isolation and identification of the canine herpesvirus\nin Mexico.
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